- · 《心脏杂志》期刊栏目设[01/26]
- · 《心脏杂志》投稿方式[01/26]
- · 心脏杂志版面费是多少[01/26]
敲除小鼠胚胎心脏流出道内第二生心区和心脏神(7)
作者:网站采编关键词:
摘要:[20] 师亮,李慧超,景雅,等.鼠胚呼吸内胚层相关第二生心区发育中的上皮-间充质转化[J].解剖学报,2018,49(4):480-485. [21] RAMIREZ A, ASTROF S. Vualization and analysis of
[20] 师亮,李慧超,景雅,等.鼠胚呼吸内胚层相关第二生心区发育中的上皮-间充质转化[J].解剖学报,2018,49(4):480-485.
[21] RAMIREZ A, ASTROF S. Vualization and analysis of pharyngeal arch arteries using whole-mount immunohistochemistry and 3D reconstruction. J Vis Exp. 2020. Doi:10.3791/.
[22] SONG YC, DOHN TE, RYDEEN AB, et al. HDAC1-mediated repression of the retinoic acid-responsive gene ripply3 promotes second heart field development. PLoS Genet. 2019;15(5):e.
[23] WITZEL HR, JUNGBLUT B, CHOE CP, et al. The LIM protein ajuba restricts the second heart field progenitor pool by regulating Isl1 activity. Dev Cell. 2012;23(1):58-70.
[24] PIVEN OO, WINATA CL. The canonical way to make a heart: β-catenin and plakoglobin in heart development and remodeling. Exp Biol and Med. 2017;242(18):1735-1745.
[25] ANTHONY J, HORTON AJ, BROOKER J, et al. Nkx2.5 second heart field target gene Ccdc117 regulates DNA metabolism and proliferation. Sci Rep. 2019;9(1):1738.
[26] COLOMBO S, DE SENA-TOMAS C, GEORGE V, et al. Nkx2.5 genes establish second heart field cardiomyocyte progenitors at the arterial pole and pattern the venous pole through Isl1 2019. Doi: 10.1242/dev..
[27] ZHOU L, LIU JL, XIANG ML, et al. Gata4 potentiates second heart field proliferation and Hedgehog signaling for cardiac septation. Proc Natl Acad Sci U S A. 2017;114(8):E1422-E1431.
[28] SAWADA H, RATERI DL, MOORLEGHEN JJ, et al. Smooth muscle cells derived from second heart field and cardiac neural crest reside in spatially distinct domains in the media of the ascending aorta-brief report. Arter Thromb Vasc Biol. 2017;37:1722-1726.
[29] MOLIN DG, POELMANN RE, DERUITER MC, et al. Transforming growth factor beta-SMAD2 signaling regulates aortic arch innervation and development. Circ Res. 2004;95(11):1109-1117.
[30] KAREN LW, CECILIA WL, MARGARET L, et al. Connexin 43 expression reflflects neural crest patterns during cardiovascular development. Dev Biol. 1999;208:307-323.
[31] XU X, FRANCIS R, WEI CJ, et al. Connexin 43-mediated modulation of polarized cell movement and the directional migration of cardiac neural crest cells. Development. 2006;133(18):321-324.
BACKGROUND:Cx43 plays an important role in human congenital heart disease. However, there is still no consistent conclusion about the formation mechanism of cardiac malformation in Cx43 knockout mouse embryos.
OBJECTIVE:To investigate the cardiac development defects and the migration and differentiation of progenitor cells of the second heart field and cardiac neural crest in Cx43 knockout mouse :Serial sections of Cx43 gene knockout homozygous (Cx43-/-) mouse and Cx43 wild-type (Cx43+/+) mouse embryos from embryonic day (ED) 10 to ED13 were made for immunohistochemical and immunofluorescent staining, and three-dimensional reconstruction of the AND CONCLUSION:(1) In Cx43 gene knockout mouse embryos at ED10-ED11, Isl1 positive second heart field cells in the ventral mesenchyme of the foregut extended through the area between the bilateral arch arteries to the dorsal wall of pericardial cavity. Meanwhile, Isl1 positive cells in the core mesenchyme of the branchial arches were continuous with those in the dorsal wall of pericardial cavity and the distal wall of the outflow tract. At ED13, the distribution of Isl1 positive cells was observed in the wall of the ascending aorta and pulmonary trunk as well as the wall of the left and right outflow tracts of the septated ventricles. However, compared with wild-type mouse embryos, fewer Isl1 positive second heart field cells were found in Cx43 gene knockout mouse embryos (P< 0.01). (2) During ED10 to ED11, Ap2α positive neural crest cells were still found in the wall of the arch artery and the dorsal and ventral walls of the aortic sac in Cx43 gene knockout mouse embryos, but the number of neural crest cells was less than that of wild-type mouse embryos (P< 0.01).(3) These results indicate that the migration path and distribution pattern of Isl1 positive second heart field cells and Ap2α positive cardiac neural crest cells are similar between the Cx43 gene knockout and wild-type mouse embryos, but the number of two kinds of migrating cells is reduced after Cx43 gene suggests that in addition to cardiac neural crest derived cells, the decrease of second heart field progenitor cells might be involved in the formation of outflow tract malformations in Cx43 knockout mouse embryos.
文章来源:《心脏杂志》 网址: http://www.xzzzzzs.cn/qikandaodu/2021/0226/424.html
上一篇:心脏细胞衰老与基因的缺失
下一篇:社区型糖尿病患者心脏自主神经病变发生情况及